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Freezing Systems

CTE – The Erlangen freezing technique

The CTE 920 is a programmable device to freeze and thaw biological material like embryos, oocytes and semen. It has especially been developed for cryopreservation in conjunction with IVF treatments.
Apart from that, the CTE 920 system is well suited for the use in other fields of medicine and biology because of its outstanding qualities concerning accuracy and reproducibility of freezing protocols.
It has been designed to meet the special requirements both in research and in routine freezing procedures.
 
 
Open-Vessel Principle

Contrary to freezing-chamber equipments the CTE 920 operates on the so called „open-vessel“ principle. This method utilizes the temperature distribution above the suface of liquid nitrogen in a thermally insulated open vessel. Cooling an warming is achieved by moving the containers which hold the cells down and up in the nitrogen vapor.

Self-Seeding

A crucial part of the Erlangen Freezing Technique is the completely new method of fully automatic induction of crystallization. The so-called „Self-Seeding“ is obtained by special freezing containers.

Self-seeding is triggered by a cristal which slowly grows upwards in the this plastic tube (tail) until it reaches the inside of the straw just when the cryoprotectant there has attained freezing temperature. Ice formation which is induced that way proceeds very slowly, causing only a slight increase of temperature of the cryoprotectant.

Temperature Control inside a straw

CTE 920 measures and controlles the temperature inside the straw. For this purpose a special control algorithm is used which was developed on the basis of highly sophisticated investigations of the thermodynamic properties of this system.
Technical request: support@mtg-de.com


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