The CTE 920 is a programmable device to freeze and thaw biological material like
embryos, oocytes and semen. It has especially been developed for cryopreservation
in conjunction with IVF treatments. Apart from that, the CTE 920 system is well suited
for the use in other fields of medicine and biology because of its outstanding
qualities concerning accuracy and reproducibility of freezing protocols. It has been
designed to meet the special requirements both in research and in routine freezing
procedures.
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Open-Vessel Principle
Contrary to freezing-chamber equipments the CTE 920 operates on the so called
„open-vessel“ principle. This method utilizes the temperature distribution above the
suface of liquid nitrogen in a thermally insulated open vessel. Cooling an warming is
achieved by moving the containers which hold the cells down and up in the nitrogen
vapor.
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Self-Seeding
A crucial part of the Erlangen Freezing Technique is the completely new method of fully
automatic induction of crystallization. The so-called „Self-Seeding“ is obtained by special
freezing containers.
Self-seeding is triggered by a cristal which slowly grows upwards in the this plastic tube
(tail) until it reaches the inside of the straw just when the cryoprotectant there has
attained freezing temperature. Ice formation which is induced that way proceeds very
slowly, causing only a slight increase of temperature of the cryoprotectant.
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Temperature Control inside a straw
CTE 920 measures and controlles the temperature inside the straw. For this purpose a
special control algorithm is used which was developed on the basis of highly sophisticated
investigations of the thermodynamic properties of this system.
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